赤霉素处理对苎麻蛋白表达调控的研究

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赤霉素处理对苎麻蛋白表达调控的研究(毕业论文14000字)
摘要：赤霉素(GA)是植物生长过程中参与调节的重要激素之一, 前期的研究表明GA对苎麻生长有重要影响。本研究旨在进一步探讨赤霉素与其对照处理对苎麻体内各物质的影响以及该影响与苎麻蛋白表达的关系，找出差异蛋白。选取湘苎2号苎麻，通过赤霉素和蒸馏水对照处理，观察与记录苎麻茎秆伸长生长的情况，统计分析所受影响的程度与显著性。测定苎麻中水溶物，果胶，半纤维素，木质素，纤维素等物质含量，分别提取各组茎皮顶芽的蛋白，做蛋白质电泳分析和免疫印记。结果表明，赤霉素对苎麻株高有显著増长作用，同时能使麻纤维中木质素和果胶含量明显增加。本研究为赤霉素信号途径的摸索打下基础，为阐明赤霉素与苎麻伸长生长之间的关系能够提供更多线索。
关键词：苎麻；赤霉素；电泳

Study of GA Treating Effects to Ramie’s Expression of Protein

Abstract: Gibberellin Acid (GA) is a kind of important hormones which involves in the regulation of plant growth. And previous studies showed that GA has an important influence on the growth of ramie. This study was designed to further study the influence of GA treatment to the growth of ramie and clarify the relationship between the influence and the expression of the protein, then find the different expression proteins. Xiangzhu2 variation of ramie was selected as object and treated by GA and the distilled water as blank control. The stems elongation were observed and recorded, and the data were statistically analyzed. Eight weeks later, the contents of water soluble substance, pectin, hemicelluloses, lignin and cellulose in ramie filament products were tested. The total protein of bark and top bud of each group were extracted, then analyzed them by electrophoresis and immuno-blotting. The results showed that GA has significantly influence to the elongation of ramie’s stem, and it can increase the contents of pectin and lignin in its fibers. All together, our results will contribute to discovery of GA signaling pathway and provide more clues for study on the relationship between GA and ramie’s stem elongation.

Key words: ramie；Gibberellin acid；electrophoresis

摘要 1

关键词 1

1 前言 2

1.1 赤霉素信号途径的研究进展 2

1.1.1 GA信号途径的主要组分 3

1.1.2 GA受体 4

1.2 赤霉素处理蛋白表达调控 4

1.2.1 DELLA 蛋白的保守结构域 5

1.2.2 DELLA 蛋白降解的分子机制 5

1.2.3 GA信号转导模式 5

1.3 用双向电泳分析赤霉素调控苎麻蛋白表达的意义 6

1.3.1 蛋白质组 6

1.3.2 双向凝胶电泳 6

1.3.3 在苎麻中寻找差异蛋白的意义 6

2 材料与方法 7

2.1 材料 7

2.1.1 试剂 7

2.1.2 仪器 7

2.1.3 材料的选定 8

2.2 实验方法 9

2.2.1 苎麻的处理方法 9

2.2.2 苎麻株高与节间距的测量 9

2.2.3 样品的提取和保存 9

2.2.4 蛋白质的提取 9

2.2.5 苎麻中各物质含量测定 10

2.2.6 蛋白质电泳和western blot 11

3 结果与分析 13

3.1 GA与对照处理对苎麻伸长生长的影响 13

3.1.1 苎麻株高与节间距的生长记录 13

3.1.2 GA处理与对照处理的株高影响分析 16

3.2 GA处理与苎麻各物质含量测定结果 16

3.3 蛋白质电泳结果及分析 17

4 结论 18

参考文献 18

致谢 20