猪血红蛋白的聚合及功能研究

猪血红蛋白的聚合及功能研究(8200字)

摘  要：本文以资源丰富的猪血为原料，利用纯化步骤获取高纯度猪血红蛋白，以PEG聚合猪血红蛋白制备聚合体。用SDS-PAGE多角度激光散射仪，鉴定了猪Hb的纯度和PEG聚合猪Hb的分子量分布。         
关键词：PEG；猪血红蛋白；血红蛋白氧载体；

Study on the polymerization and function of porcine hemoglobin
 
Abstract: Using pig blood as the raw material, ultra-pure porcine hemoglobin was obtained through multiple purification steps; with PEG as the cross-linker, polymerized porcine hemoglobin (pPolyHb) was then produced. Various physical and chemical properties, such as purity of the purified pHb, oxygen affinity and auto-oxidation tendency of the proteins were analyzed.
Key words: PEG;hemoglobin ; procine hemoglobin-based oxygen carriers

目  录
摘要……………………………………………………………………………1
关键词…………………………………………………………………………1
1前言…………………………………………………………………………2
1.1细胞代用品研究概况……………………………………………………2
1.2血红蛋白类血液代用品概况……………………………………………2
1.2.1血红蛋白的结构………………………………………………………2
1.2.2血红蛋白的生理功能…………………………………………………4
    1.2.3红蛋白氧亲和力的调控………………………………………………5
1.2.4血红蛋白类氧载体(HBOCs) …………………………………………5
1.3天然血红蛋白直接作为血液代用品的缺陷……………………………6
1.4血液替代品研究常用方法………………………………………………6
2材料与方法…………………………………………………………………7
2.1材料………………………………………………………………………7
2.1.1主要试剂………………………………………………………………7
    2.1.2主要仪器………………………………………………………………7
2.2方法………………………………………………………………………7
2.2.1无菌采集新鲜猪血……………………………………………………7
2.2.2清洗血液并提取血红蛋白……………………………………………7
2.2.3配制水液两相系统聚合血红蛋白……………………………………7
2.2.4透析……………………………………………………………………8
2.2.5PEG聚合猪血红蛋白pH值测定………………………………………8
2.2.6聚丙烯酰胺凝胶电泳(SDS-PAGE)测定PEG聚合猪血红蛋白纯度…8
3结果…………………………………………………………………………9
    4讨论…………………………………………………………………………10
参考文献………………………………………………………………………11
致谢……………………………………………………………………………11