病死猪肉的快速检测方法

病死猪肉的快速检测方法(5000字)

摘  要：本试验研制了的病死猪肉快速检测试纸，用过氧化物酶反应法、硫酸铜肉浸液法反应法、pH试纸法和自制试纸检测法对53份样品猪肉进行肉浸液的提取，通过测定两组猪肉中过氧化物酶的含量，来判定所检测的样品是否为病死猪肉。病死猪肉的快速检测试纸相对于目前常用的检测方法有操作简单，显示结果迅速，方便大批量病死猪肉的检测等明显优势。病死猪肉是危害动物性食品安全、影响人民健康的一类肉品，研究其快速检测方法可以为市场肉品检疫提供较好的工具，可以方便地在对病死猪肉进行宰后检疫。
关键词：病死猪肉；过氧化物酶；检测试纸

RAPID DERECTION OF DISEASED PORK

Abstract:In this experiment has produced a good test strip pairs for 53 samples of pork meat extract in the extraction, by measuring the two groups of pork in peroxidase concentration, to determine whether the test samples of diseased pigs. Dead pork test strip relative to the currently used detection methods is simple to show results quickly, easily mass detection of diseased pigs obvious advantages. Dead animal meat is a food safety hazard, affecting people's health in a class of meat, of the rapid test for the market to provide better tools for meat quarantine can be easily carried out on dead meat after slaughter and quarantine.
Key words: Diseased pork; peroxidase; test strip
 

目    录
 
摘  要    1
关键词    1
1  前言    1
2  实验材料与方法    3
2.1  材料    3
2.1.1肉样来源    3
2.1.2试剂    3
2.1.3器材及设备    3
2.2 实验方法    3
2.2.1猪肉肉浸液的提取    3
2.2.2病死猪肉试纸检测法    4
2.2.3硫酸铜肉浸液法    4
2.2.4过氧化物酶试剂法    4
2.2.5 pH试纸法    5
3 实验结果    5
3.1病死猪肉试纸检测法检测结果    5
3.2硫酸铜肉浸液法检测结果    6
3.3过氧化物酶试剂法检测结果    6
3.4 pH试纸法检测结果    7
4 讨论    7
4.1六种检测方法的优缺点对比    7
4.2实验过程中的难点    7
4.2.1试纸的稳定性    7
4.2.2检测溶液浓度比的配制    8
4.3快速检测试纸条的创新点    8
4.3.1    8
4.3.2    8
4.3.3    8
4.3.4    8
5 结论    8
参考文献    9
致    谢    9