三聚氰胺及三聚氰酸混合物对小鼠肝脏损伤的SOD、ALT 和AST活性变

三聚氰胺及三聚氰酸混合物对小鼠肝脏损伤的SOD、ALT 和AST活性变化的观察(8800字)

摘  要：目的：实验主要是通过一些常规方法检测，研究三聚氰胺和三聚氰酸对小鼠肝脏的损伤情况，以及探讨相关的一些损伤机理。为人类及动物食品安全生产提供科学有效的理论依据。方法：清洁级昆明系雄性小白鼠60只；将小白鼠随机分成6组,每组10只,采取隔天灌胃的方式给小白鼠攻毒，实验30d，取出肝脏制作肝脏组织匀浆；测定各项生化指标。结果：SOD活性下降和MDA含量升高；血清中ALT和AST随着三聚氰胺和三聚氰酸混合物剂量的升高而明显上升。结论：三聚氰胺和三聚氰酸混合物能影响小鼠肝功能和损伤小鼠肝脏细胞，且随混合物剂量的增加，呈一定的量效关系，高剂量混合物组的小鼠肝脏损伤最为明显。而单独的三聚氰胺和三聚氰酸对小鼠肝脏损伤不明显。
关键词：三聚氰胺；三聚氰酸；SOD；ALT；AST

The observation of SOD、 ALT and AST activity of liver in mice which feeding with melamine and cyanuric acid mixture

Abstract: Objective: This study is mainly detected by some conventional methods，for investigating melamine and cyanuric acid on liver injury in mice，and to explore some related damage mechanism。 It's to provide scientific and effective theory for human and animal food safety. Methods: 60 clean grade Male Kunming mice The mice were randomly divided into 6 groups, 10 each group. Take the next day administered to mice toxic attack the control group (KD group), melamine and cyanuric acid mixed with low, medium and high dose group (HD group, HZ group, HG group), it should be experimented for 30 days. Remove the liver and make the liver tissue; determine the biochemical indicators. Results: SOD activity decreased and the MDA content increased; The ALT and AST in the serum has a obvious increase with the rise of dose melamine and cyanuric acid mixture . Conclusion: The mixture of melamine and cyanuric acid can affect liver function and injury in mouse liver cells, and increasing with the dose of the mixture, showing dose-respones relationship, and high dose mixture group has a most obvious  injury in mice liver. The separate melamine or cyanuric acid has a minor effect on mice liver.
Key words: Melamine; Cyanuric acid;SOD;ALT;AST
 

目    录
摘   要    1
关键词    1
1 前言    2
1.1 研究背景    2
1.2 三聚氰胺及三聚氰酸    2
1.2.1 三聚氰胺的理化性质及代谢    2
1.2.2 三聚氰酸的理化性质及代谢    3
1.2.3 三聚氰胺及三聚氰酸的毒性    4
1.3 本研究的目的及意义    4
2 材料及方法    5
2.1材料    5
2.1.1 主要实验仪器设备及供试材料    5
2.1.2 试验动物    5
2.1.3 试验药品    5
2.1.4 试验试剂    5
2.2 实验方法与试验设计    5
2.2.1 实验动物    5
2.2.2 试剂剂量选定    5
2.2.3 动物分组及给药方式    5
2.3 各项试剂指标的测定    6
2.3.1 总蛋白（TP）含量测定：（双缩脲法）    6
2.3.2 超氧化物歧化酶（SOD）活性测定    6
2.3.3 丙二醛（MDA）含量测定    7
2.3.4 血清ALT活性测定    7
2.3.5 血清AST活性测定    7
2.3.6 统计学处理    8
3 结果与分析    8
3.1血清总蛋白（TP）含量测定结果    8
3.2 SOD活性测定结果    9
3.3 MDA含量测定结果    9
3.4 谷丙转氨酶（ALT）活性的测定结果    9
3.5 天门冬氨酸转移酶（AST）活性的测定结果    10
4  讨论    10
4.1 三聚氰胺及三聚氰酸对小鼠相关血液生化指标的影响    10
4.2 三聚氰胺及三聚氰酸对小鼠肝功能的影响    11
5 结论    12
参考文献    12
致    谢    14